Journal: The FEBS journal

Article Title: Variable cytoplasmic actin expression impacts the sensitivity of different dystrophin-deficient mdx skeletal muscle to eccentric contraction

doi: 10.1111/febs.14831

Figure Lengend Snippet: (A) Representative immunohistochemical myosin heavy chain stains (MHC), (B) MHC type I, IIa, IIx and IIb quantification, (C) muscle fiber type cross-sectional area, (D) utrophin expression relative to EDL, (E) representative H&E stains, (F) central nucleated fiber quantification, (G) representative fibrosis stain and (H) fibrosis quantification for mdx EDL, PL and Sol muscle. * Different from EDL, # different from PL at p < 0.05. n ≥ 4/group except Fig. 2C where all fiber cross-sections were measured up to 120 fibers of a single type. EDL: Extensor Digitorum Longus, PL: Peroneus Longus, Sol: Soleus.

Article Snippet: Sequential staining, as described above, was then performed with MHC IIA primary antibody (ATCC, Manassas, VA, Cat#HB-277, 1:40) followed by Streptavidin-Texas Red secondary antibody (Vector Laboratories, Burlingame, CA, Cat#SA-5006, 1:60) and MHC I primary antibody (Developmental Studies Hybridoma Bank, University of Iowa, Cat#BA-F8, 1:20) followed by Streptavidin-AMCA secondary antibody (Vector Laboratories, Burlingame, CA, Cat#SA-5008, 1:60).

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: The Journal of Cell Biology

Article Title: Different modes of hypertrophy in skeletal muscle fibers

doi: 10.1083/jcb.200105147

Figure Lengend Snippet: Trend in fiber type changes in gracilis anterior and posterior muscle influenced by exercise not transgene. (A) Gracilis anterior muscle showed a trend of increasing type IIB MyHC at the cost of type IIA/X MyHC–positive fibers. A significant difference was only found in WT-EX and transgenic mice. (B) Fiber type changes in gracilis posterior muscle showed a trend toward type IIA/X MyHC–positive fibers at the cost of fibers expressing type IIB and type I MyHC. Exercise appears to be the main influence on these trends.

Article Snippet: The sections were incubated with either anti-MyHC I antibody (American Type Culture Collection A4.951; 1:10 dilution in PBS/1% BSA) or anti-MyHC IIB antibody (American Type Culture Collection BF-F3; 1:10 dilution in PBS/1% BSA), washed three times in PBS, and incubated in secondary antibody (Jackson ImmunoResearch Laboratory; peroxidase-conjugated goat anti–mouse IgG 115-035-164, or peroxidase-conjugated goat anti–mouse IgM 115-035-075, both 1:500 in PBS/1% BSA).

Techniques: Transgenic Assay, Expressing